Labosky P A, Kaestner K H
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 36th and Hamilton Walk, Philadelphia, PA 19104-6058, USA.
Mech Dev. 1998 Aug;76(1-2):185-90. doi: 10.1016/s0925-4773(98)00105-1.
The embryonic neural crest is a unique group of cells that gives rise to the peripheral nervous system as well as many other cell types. Most of the work describing the behavior and specification of these cells has been done in the avian system, a system amenable to experimental manipulations such as tissue grafting, cell transplantation and lineage tracing. This work has been greatly facilitated by the use of molecular markers of neural crest cells such as HNK-1 and slug, markers that are not yet available for the study of mouse neural crest. Here we demonstrate that Hfh2 (HNF3 forkhead homologue 2), a member of the 'winged helix' or 'forkhead' transcription factor gene family, is expressed in premigratory and migrating neural crest cells in the early mouse embryo and in motorneuron progenitors in the developing spinal cord. Using linkage analysis we have localized the Hfh2 gene to chromosome 4 at 44.91 cM from the centromere.
胚胎神经嵴是一组独特的细胞，可产生外周神经系统以及许多其他细胞类型。描述这些细胞行为和特化的大部分工作是在鸟类系统中完成的，该系统适用于组织移植、细胞移植和谱系追踪等实验操作。神经嵴细胞分子标记物如HNK-1和蛞蝓蛋白的使用极大地促进了这项工作，而这些标记物在小鼠神经嵴研究中尚不可用。在这里，我们证明Hfh2（HNF3叉头同源物2）是“翼状螺旋”或“叉头”转录因子基因家族的成员，在小鼠早期胚胎的迁移前和迁移神经嵴细胞以及发育中的脊髓运动神经元祖细胞中表达。通过连锁分析，我们已将Hfh2基因定位到4号染色体上，距离着丝粒44.91 cM处。
