Nakatani C, Muramatsu T, Shirai T
Department of Dermatology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
Br J Dermatol. 1998 Sep;139(3):365-70. doi: 10.1046/j.1365-2133.1998.02396.x.
The 180 kDa bullous pemphigoid (BP) antigen (BP180) is known to be recognized by sera from patients with BP, herpes gestationis (HG) and cicatricial pemphigoid (CP). A series of previous studies using BP180 recombinant proteins has shown that most sera from patients with BP and HG react with the NC16A domain of BP180, an extracellular non-collagenous region just adjacent to the plasma membrane. In contrast, the C-terminal region of BP180 has been reported to be one of the epitopes of CP. In the present study, we examined the immunoreactivity of 110 BP sera against the NC16A and C-terminal domains of BP180 using immunoblot analysis and enzyme-linked immunosorbent assay (ELISA). Immunoblot analysis revealed that 100 (91%) and 26 (23.5%) of the 110 BP sera recognized the NC16A and C-terminal domains, respectively. The results of the ELISA were correlated with those of immunoblotting. There were no specific or significant clinical features such as severe involvement of mucous membranes and scarring in BP patients whose sera reacted with the C-terminal region. These findings suggest that some BP sera react with the C-terminal region of BP180 without any association with the characteristic clinical features of CP.
已知180 kDa大疱性类天疱疮（BP）抗原（BP180）可被BP、妊娠疱疹（HG）和瘢痕性类天疱疮（CP）患者的血清识别。此前一系列使用BP180重组蛋白的研究表明，大多数BP和HG患者的血清与BP180的NC16A结构域发生反应，该结构域是紧邻质膜的细胞外非胶原区域。相比之下，BP180的C末端区域据报道是CP的表位之一。在本研究中，我们使用免疫印迹分析和酶联免疫吸附测定（ELISA）检测了110份BP血清对BP180的NC16A和C末端结构域的免疫反应性。免疫印迹分析显示，110份BP血清中分别有100份（91%）和26份（23.5%）识别NC16A和C末端结构域。ELISA结果与免疫印迹结果相关。血清与C末端区域发生反应的BP患者没有如严重黏膜受累和瘢痕形成等特定或显著的临床特征。这些发现表明，一些BP血清与BP180的C末端区域发生反应，且与CP的特征性临床特征无关。
