Badovinac V, Boggiano C, Trajković V, Frey A B, Vujanović N L, Gold D P, Mostarica-Stojković M, Vukmanović S
Institute for Biological Research, Belgrade, Yugoslavia.
Immunology. 1998 Sep;95(1):117-25. doi: 10.1046/j.1365-2567.1998.00567.x.
Cells expressing markers of both natural killer and T lymphocytes (NK T cells) in humans and mice express a restricted T-cell receptor (TCR) repertoire, are of CD4- CD8- or CD4+ CD8- phenotype, and upon anti-CD3 stimulation secrete large amounts of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma). NK T cells may be the primary source of IL-4-promoting T helper type 2 (Th2) responses and/or they might be involved in regulating the balance between Th1- and Th2-type immune responses, and may consequently affect susceptibility to autoimmune diseases associated with a skewed Th phenotype. We show that rat NK T cells selectively proliferate to IL-2, and use this fact to analyse cytokine production by NK T cells in two rat strains differentially susceptible to Th1- or Th2-type autoimmune diseases. Analysis by reverse transcription-polymerase chain reaction revealed that, in contrast to mouse, rat NK T cells secrete exclusively IFN-gamma and not IL-4 after anti-CD3 stimulation, and use a wider TCR-Vbeta repertoire, suggesting that rat NK T cells are not essential for the development of Th2-type CD4+ T-cell responses.
在人类和小鼠中，表达自然杀伤细胞和T淋巴细胞标志物的细胞（自然杀伤T细胞）表达受限的T细胞受体（TCR）库，具有CD4 - CD8 - 或CD4 + CD8 - 表型，并且在抗CD3刺激后分泌大量白细胞介素 - 4（IL - 4）和干扰素 - γ（IFN - γ）。自然杀伤T细胞可能是促进白细胞介素 - 4的辅助性T细胞2型（Th2）反应的主要来源，和/或它们可能参与调节Th1型和Th2型免疫反应之间的平衡，因此可能影响与Th表型偏斜相关的自身免疫性疾病的易感性。我们发现大鼠自然杀伤T细胞对IL - 2有选择性增殖，并利用这一事实分析了两种对Th1型或Th2型自身免疫性疾病易感性不同的大鼠品系中自然杀伤T细胞的细胞因子产生情况。逆转录 - 聚合酶链反应分析显示，与小鼠不同，大鼠自然杀伤T细胞在抗CD3刺激后仅分泌IFN - γ而不分泌IL - 4，并且使用更广泛的TCR - Vβ库，这表明大鼠自然杀伤T细胞对于Th2型CD4 + T细胞反应的发展并非必不可少。
