Kim W S, Khunajakr N, Ren J, Dunn N W
Department of Biotechnology, The University of New South Wales, Sydney, NSW 2052, Australia.
Curr Microbiol. 1998 Nov;37(5):333-6. doi: 10.1007/s002849900387.
Primers designed from consensus regions of the major cold shock gene of different bacterial species were used in PCR amplification of Lactic Acid Bacteria (LAB). An appropriately-sized PCR product was obtained from Lactococcus lactis subsp. lactis LL43-1 and MG1363; Lactococcus lactis subsp. cremoris LC10-1, LC11-1, and LC12-1; Streptococcus thermophilus ST1-1; Enterococcus faecalis EF1-1; Lactobacillus acidophilus LA1-1; Lactobacillus helveticus LH1-1; Pediococcus pentosaceus PP1-1; and Bifidobacterium animalis BA1-1. The PCR products were cloned and sequenced. The deduced amino acid sequences displayed high sequence similarity with the major cold shock proteins of Escherichia coli and Bacillus subtilis and the human Y-box factor. The amino acid residues of the cold shock domain implicated in nucleic acid binding in several unrelated species were also highly conserved in the LAB strains. It is possible, therefore, that this protein in LAB may also act as a transcriptional enhancer to other cold shock genes and/or act as an RNA chaperone unwinding tightly folded RNA molecules.
从不同细菌物种的主要冷休克基因的共有区域设计的引物，用于乳酸菌（LAB）的PCR扩增。从乳酸乳球菌乳酸亚种LL43 - 1和MG1363；乳酸乳球菌乳脂亚种LC10 - 1、LC11 - 1和LC12 - 1；嗜热链球菌ST1 - 1；粪肠球菌EF1 - 1；嗜酸乳杆菌LA1 - 1；瑞士乳杆菌LH1 - 1；戊糖片球菌PP1 - 1；以及动物双歧杆菌BA1 - 1中获得了大小合适的PCR产物。对PCR产物进行克隆和测序。推导的氨基酸序列与大肠杆菌和枯草芽孢杆菌的主要冷休克蛋白以及人类Y盒因子显示出高度的序列相似性。在几个不相关物种中涉及核酸结合的冷休克结构域的氨基酸残基在LAB菌株中也高度保守。因此，LAB中的这种蛋白质可能也作为其他冷休克基因的转录增强子和/或作为解开紧密折叠的RNA分子的RNA伴侣发挥作用。
