Saha BC, Bothast RJ
Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U. S. Department of Agriculture, Peoria, IL 61604, USA.
Curr Microbiol. 1998 Nov;37(5):337-40. doi: 10.1007/s002849900388.
A color-variant strain of Aureobasidium pullulans (NRRL Y-12974) produced alpha-L-arabinofuranosidase (alpha-L-AFase) when grown in liquid culture on sugar beet arabinan, wheat arabinoxylan, L-arabinose, L-arabitol, xylose, xylitol, oat spelt xylan, corn fiber, or arabinogalactan. L-Arabinose was most effective for production of both whole-broth and extracellular alpha-L-AFase activity, followed by L-arabitol. Oat spelt xylan, sugar beet arabinan, xylose, xylitol, and wheat arabinoxylan were intermediate in their ability to support alpha-L-AFase production. Lower amounts of enzyme activity were detected in corn fiber- and arabinogalactan-grown cultures.
在以甜菜阿拉伯聚糖、小麦阿拉伯木聚糖、L-阿拉伯糖、L-阿拉伯糖醇、木糖、木糖醇、燕麦麸木聚糖、玉米纤维或阿拉伯半乳聚糖为培养基的液体培养物中生长时，出芽短梗霉的一个颜色变异菌株（NRRL Y-12974）会产生α-L-阿拉伯呋喃糖苷酶（α-L-AFase）。L-阿拉伯糖对全发酵液和细胞外α-L-AFase活性的产生最为有效，其次是L-阿拉伯糖醇。燕麦麸木聚糖、甜菜阿拉伯聚糖、木糖、木糖醇和小麦阿拉伯木聚糖在支持α-L-AFase产生的能力方面处于中等水平。在以玉米纤维和阿拉伯半乳聚糖为培养基的培养物中检测到的酶活性较低。
