[用于检测轮状病毒抗原的斑点酶联免疫吸附测定法的评估]
[Evaluation of a Dot ELISA for the detection of a Rotavirus antigen].
作者信息
Ribas Antúnez M A, Fernández D, Rodriguez C, Toraño I, Pimentel T
机构信息
Instituto de Medicina Tropical Pedro Kouri.
出版信息
Rev Cubana Med Trop. 1996;48(1):59-61.
PMID:9768273
Abstract
An evaluation of a Dot ELISA made by the Center of Genetic Engineering and Biotechnology for detecting Rotavirus antigen, was carried out. 100 feces samples were analyzed by this technique and the results were compared with those obtained with polyacrylamide gel electrophoresis, technique traditionally used in the Rotavirus diagnosis. It was obtained a high coincidence, specificity and sensitivity among them.
摘要
对基因工程与生物技术中心生产的用于检测轮状病毒抗原的斑点酶联免疫吸附测定法进行了评估。采用该技术对100份粪便样本进行了分析，并将结果与轮状病毒诊断中传统使用的聚丙烯酰胺凝胶电泳技术所获得的结果进行了比较。结果发现二者具有高度的一致性、特异性和敏感性。
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