Georgakilas A G, Sakelliou L, Sideris E G, Margaritis L H, Sophianopoulou V
Institute of Biology, National Center for Scientific Research DEMOKRITOS, Agia Paraskevi, Athens, Greece.
Radiat Res. 1998 Oct;150(4):488-91.
The effects of gamma radiation on the stability and size of mammalian DNA were studied by using thermal transition spectrophotometry and pulsed-field and standard agarose gel electrophoresis. The experiments were performed using deproteinized calf thymus DNA in buffered deaerated aqueous solutions. A dual dose response was observed: a tendency for increased helix stability at "low" doses (0-4 Gy) accompanied by a high tendency of the DNA molecules to interact, forming larger molecules, followed by a gradual increase of degradation and helix instability at higher doses. The results reported here for the low-dose region are consistent with the hypothesis of inter- and intramolecular interactions of covalent character (crosslinking) in irradiated DNA molecules.
通过热转变分光光度法以及脉冲场和标准琼脂糖凝胶电泳研究了γ辐射对哺乳动物DNA稳定性和大小的影响。实验采用在缓冲脱气水溶液中的脱蛋白小牛胸腺DNA进行。观察到双剂量反应：在“低”剂量（0 - 4 Gy）下螺旋稳定性增加的趋势，同时DNA分子相互作用形成更大分子的趋势很高，随后在较高剂量下降解和螺旋不稳定性逐渐增加。此处报道的低剂量区域的结果与辐照DNA分子中具有共价性质的分子间和分子内相互作用（交联）的假设一致。
