Jones G V, Botham C A, Clarke A G, Kendall M D
The Babraham Institute, Babraham Hall, Babraham, Cambridge, CB2 4AT, United Kingdom.
Brain Behav Immun. 1998 Sep;12(3):163-80. doi: 10.1006/brbi.1998.0528.
In order to study the functional development of a thymus in an experimental model, small pieces of adult rat thymic tissue were cultured for 9 days and implanted under the kidney capsule of littermates. The tissues were examined with a panel of antibodies raised against thymic and neural factors and neural crest cells at intervals from 5 to 13 days. At 5 days post-implantation, there were groups of L1+ cells within the implants that reacted with antibodies raised against neural and neural crest cell markers. L1+ cells were highly mitotic, rounded cells measuring 8.7 +/- 0.6 micrometer in diameter. Double immunostaining with different combinations of antibodies showed that 94% of the L1+ cells were also TH+, and many were HNK-1/NCAM+, PGP 9.5+, NGF+, chromogranin A+, VIP+, S100+, CGRP+, GAD+, and A2B5+. A few were also pan-cytokeratin+. These results indicate that these cells are derived from neural crest derived cells and belong to the neuroepithelial line of development. The L1+ cells were most numerous before nerves appeared (about Day 9) and reduced in number and extent as the thymus differentiated. The neural crest cells occasionally had long cytoplasmic extensions, but it was not possible to decide if they formed the nerves that appeared in the implants. Adult thymuses also contained a population of L1+ and HNK-1/NCAM+ cells, mainly in the subcapsular cortex, the septa, and the medulla. These cells could be a source of neural crest cells able to repopulate the implant. The adult thymus may always contain a reservoir of cells potentially capable of producing neuropeptides and transmitter factors required for thymic growth and regeneration.
为了在实验模型中研究胸腺的功能发育，将成年大鼠胸腺组织小块培养9天，然后植入同窝仔鼠的肾被膜下。在植入后的5至13天内，间隔使用一组针对胸腺、神经因子和神经嵴细胞产生的抗体对组织进行检查。植入后5天，植入物内有一群L1 +细胞，它们与针对神经和神经嵴细胞标志物产生的抗体发生反应。L1 +细胞是高度有丝分裂的圆形细胞，直径为8.7±0.6微米。用不同抗体组合进行双重免疫染色显示，94%的L1 +细胞也是TH +，许多细胞还呈HNK - 1/NCAM +、PGP 9.5 +、NGF +、嗜铬粒蛋白A +、VIP +、S100 +、降钙素基因相关肽+、谷氨酸脱羧酶+和A2B5 +。少数细胞也呈泛细胞角蛋白+。这些结果表明这些细胞来源于神经嵴衍生细胞，属于神经上皮发育谱系。L1 +细胞在神经出现之前（约第9天）数量最多，随着胸腺分化，数量和范围减少。神经嵴细胞偶尔有长的细胞质延伸，但无法确定它们是否形成了植入物中出现的神经。成年胸腺也含有一群L1 +和HNK - 1/NCAM +细胞，主要位于被膜下皮质、间隔和髓质。这些细胞可能是能够重新填充植入物的神经嵴细胞的来源。成年胸腺可能始终含有一个细胞库，这些细胞可能能够产生胸腺生长和再生所需的神经肽和递质因子。
