Okumura A, Tokuda Y, Tanaka M, Makuuchi H, Tajima T
Department of Surgery, Tokai University School of Medicine, Kanagawa, Japan.
Jpn J Clin Oncol. 1998 Aug;28(8):480-5. doi: 10.1093/jjco/28.8.480.
Contamination of bone marrow and peripheral blood stem cells with tumor cells is a problem that may be encountered when autologous hematopoietic stem cell transplantation is conducted concurrently with high-dose chemotherapy.
Using monoclonal antibodies to a variety of tumors, the detection of tumor cells in the bone marrow of breast cancer patients was studied by immunohistochemistry.
KL-1 and CAM5.2 were strongly reactive with breast cancer cells, but not with normal bone marrow cells. The reactivity of the tumor cells with EMA was not strong, and DF-3 and 115D8 yielded only slightly positive reactions. These latter antibodies also exhibited some reactivity to normal bone marrow cells. When tumor cells were admixed with normal cells, the sensitivity of CAM5.2 and EMA permitted the detection of one cell in 10(4), but with KL-1, the detection of one in 10(5) cells was possible. When immunohistochemical staining was used in testing 40 patients with advanced or recurrent breast cancer, positive reactions were obtained in four of 27 patients (14.8%) with KL-1, four of 26 (15.4%) with CAM5.2, and nine of 37 (23.7%) with KL-1 + CAM5.2, figures similar to those reported by others who studied stage IV patients.
Immunohistochemical staining with KL-1 and CAM5.2 is therefore considered to be a useful technique for detecting contamination by tumor cells.
自体造血干细胞移植与大剂量化疗同时进行时，骨髓和外周血干细胞被肿瘤细胞污染是一个可能会遇到的问题。
使用针对多种肿瘤的单克隆抗体，通过免疫组织化学研究乳腺癌患者骨髓中肿瘤细胞的检测情况。
KL-1和CAM5.2与乳腺癌细胞有强烈反应，但与正常骨髓细胞无反应。肿瘤细胞与EMA的反应不强，DF-3和115D8仅产生弱阳性反应。后两种抗体对正常骨髓细胞也有一些反应。当肿瘤细胞与正常细胞混合时，CAM5.2和EMA的敏感性允许检测出10⁴个细胞中的1个，但使用KL-1时，可以检测出10⁵个细胞中的1个。在检测40例晚期或复发性乳腺癌患者时，27例患者中有4例（14.8%）使用KL-1检测呈阳性反应，26例中有4例（15.4%）使用CAM5.2检测呈阳性反应，37例中有9例（23.7%）使用KL-1 + CAM5.2检测呈阳性反应，这些数字与其他研究IV期患者的报告相似。
因此，KL-1和CAM5.2免疫组织化学染色被认为是检测肿瘤细胞污染的有用技术。
