Mereghetti L, Marquet-van der Mee N, Loulergue J, Rolland J C, Audurier A
Laboratoire de Bactériologie et d'Hygiène Hospitalière, Hôpital Trousseau, CHU de Tours, France.
Pathol Biol (Paris). 1998 May;46(5):319-24.
Pseudomonas aeruginosa is the most important bacterial pathogen in lung disease of cystic fibrosis patients. Different morphotypes of the bacterium are frequently recovered in sputum samples of these patients. We developed a whole cell Randomly Amplified Polymorphic DNA (RAPD) technique in order to establish the relatedness between morphotype, genotype and antibiotic susceptibility. Six cystic fibrosis patients already colonized by P. aeruginosa were investigated by collecting three successive sputum samples (before and after antibiotic treatment, and one month later) and selecting 10 isolates per morphotype. 250 isolates of P. aeruginosa were recovered from 16 of 18 sputum samples. Five patients carried a single RAPD type strain four of which showed at least two morphotypes; one patient carried two RAPD types strains. No patients carried the same strain. These results confirmed other studies previously published in showing stability of the chronic colonization with a single strain. Antibiotype differences were not associated with differences of RAPD profiles and no relation was found between antibiotype and morphotype.
铜绿假单胞菌是囊性纤维化患者肺部疾病中最重要的细菌病原体。在这些患者的痰液样本中经常发现该细菌的不同形态类型。我们开发了一种全细胞随机扩增多态性DNA（RAPD）技术，以确定形态类型、基因型和抗生素敏感性之间的相关性。通过收集6名已被铜绿假单胞菌定植的囊性纤维化患者连续的三份痰液样本（抗生素治疗前后以及一个月后），并对每个形态类型选择10株分离株，对其进行了研究。从18份痰液样本中的16份中分离出250株铜绿假单胞菌。5名患者携带单一RAPD类型菌株，其中4名至少表现出两种形态类型；1名患者携带两种RAPD类型菌株。没有患者携带相同的菌株。这些结果证实了先前发表的其他研究，表明单一菌株慢性定植具有稳定性。抗生素型差异与RAPD图谱差异无关，且未发现抗生素型与形态类型之间存在关联。
