Nazik Hasan, Ongen Betigül, Erturan Zayre, Salcioğlu Melek
Department of Microbiology and Clinical Microbiology, Istanbul University, Istanbul, Turkey.
Jpn J Infect Dis. 2007 May;60(2-3):82-6.
The purpose of this study was to type the Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates recovered from cystic fibrosis (CF) patients by random amplified polymorphic DNA (RAPD)-PCR and to determine the antibiotic susceptibility of these strains. P. aeruginosa (n=49), and S. maltophilia (n=11) isolates which had been recovered from 16 and 8 patients, respectively, during a 1-year period were investigated. Three primers were used for RAPD-PCR typing. Antibiotic susceptibility testing of all isolates was performed by the disc diffusion method. RAPD-PCR analysis revealed 21 (P. aeruginosa) and 9 (S. maltophilia) different genotypes. According to the antimicrobial susceptibility results, the P. aeruginosa and S. maltophilia strains were cumulated into 24 and 11 groups, respectively. The CF patients were colonized or infected with P. aeruginosa strains of single or sometimes multiple genotypes which remained stable over several months. Our results also revealed that cross-colonization might be possible among the patients who are followed up at the same center. Piperacillin-tazobactam for P. aeruginosa and trimethoprim-sulfamethoxazole for S. maltophilia were found to be the most active antibiotics according to our results.
本研究的目的是通过随机扩增多态性DNA（RAPD）-PCR对从囊性纤维化（CF）患者中分离出的铜绿假单胞菌和嗜麦芽窄食单胞菌进行分型，并确定这些菌株的抗生素敏感性。对在1年期间分别从16例和8例患者中分离出的49株铜绿假单胞菌和11株嗜麦芽窄食单胞菌进行了研究。使用三种引物进行RAPD-PCR分型。所有分离株的抗生素敏感性测试采用纸片扩散法进行。RAPD-PCR分析揭示了21种（铜绿假单胞菌）和9种（嗜麦芽窄食单胞菌）不同的基因型。根据抗菌药敏结果，铜绿假单胞菌和嗜麦芽窄食单胞菌菌株分别被归归为24和11个组。CF患者被单一或有时多种基因型的铜绿假单胞菌菌株定植或感染，这些基因型在几个月内保持稳定。我们的结果还表明同一中心随访的患者之间可能发生交叉定植。根据我们的结果，哌拉西林-他唑巴坦对铜绿假单胞菌和甲氧苄啶-磺胺甲恶唑对嗜麦芽窄食单胞菌是最有效的抗生素。
