Takashima A, Kitajima T
Department of Dermatology, University of Texas Southwestern Medical Center, Dallas 75235-9069, USA.
Pathol Biol (Paris). 1998 Jan;46(1):53-60.
Dendritic cells (DC) are a special subset of leukocytes characterized by their potent ability to activate immunologically naive T cells. We established a series of long-term DC lines, termed "XS lines", from the epidermis of newborn mouse skin. These lines retain many important features of resident DC in epidermis, i.e., Langerhans cells (LC), including surface phenotype, antigen (Ag) presenting capacity, and cytokine and cytokine receptor profiles. Working with XS lines, we have observed recently that DC undergo rapid and profound changes during Ag presentation. Specifically, upon Ag-specific interaction with T cell clones, XS52 cells: a) begin to secrete several cytokines (IL-1 beta, IL-6, and TNF alpha), b) lose the expression of CSF-1 receptor and proliferative responsiveness to CSF-1, c) elevate the expression of B7-2 (CD86), and d) lose the phagocytotic and adhesive capacities. We propose that these changes represent "T cell-mediated terminal maturation" of DC, a critical transition into a fully professional Ag presenting cell.
树突状细胞（DC）是白细胞的一个特殊亚群，其特征在于具有激活免疫原性幼稚T细胞的强大能力。我们从新生小鼠皮肤表皮建立了一系列长期DC系，称为“XS系”。这些细胞系保留了表皮中驻留DC的许多重要特征，即朗格汉斯细胞（LC）的特征，包括表面表型、抗原（Ag）呈递能力以及细胞因子和细胞因子受体谱。利用XS系进行研究时，我们最近观察到DC在Ag呈递过程中会发生快速而深刻的变化。具体而言，在与T细胞克隆进行Ag特异性相互作用后，XS52细胞：a）开始分泌多种细胞因子（IL-1β、IL-6和TNFα），b）失去CSF-1受体的表达以及对CSF-1的增殖反应性，c）提高B7-2（CD86）的表达，d）失去吞噬和黏附能力。我们认为这些变化代表了DC的“T细胞介导的终末成熟”，这是向完全成熟的专业Ag呈递细胞的关键转变。
