Fang W, Luther D A, Oliver S P
Department of Animal Science, University of Tennessee, Knoxville 37996, USA.
FEMS Microbiol Lett. 1998 Sep 15;166(2):237-42. doi: 10.1111/j.1574-6968.1998.tb13896.x.
Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by electrophoresis. There were proteins induced or having increased expression in both supernatant and surface-associated samples from S. uberis co-cultured with MAC-T cells. Some of these proteins were recognized by antibodies in serum obtained from a cow infected by S. uberis. In supernatant samples, there were two distinct protein bands at 35 and 36.8 kDa for all three strains of S. uberis co-cultured with MAC-T cells. These two bands were absent when bacterial protein synthesis was inhibited by chloramphenicol. This study clearly indicates that bacterial protein expression was regulated in response to co-culture with mammary epithelial cells.
从患乳腺炎的奶牛中分离出的三株乳房链球菌，在不含胎牛血清的杜尔贝科改良伊格尔培养基中，与牛乳腺上皮细胞系（MAC-T）进行共培养。通过电泳比较不同处理组培养上清液和细菌沉淀中的蛋白质谱。与MAC-T细胞共培养的乳房链球菌的上清液和表面相关样本中均有诱导产生或表达增加的蛋白质。其中一些蛋白质能被感染乳房链球菌的奶牛血清中的抗体识别。在上清液样本中，与MAC-T细胞共培养的所有三株乳房链球菌在35 kDa和36.8 kDa处有两条明显的蛋白带。当用氯霉素抑制细菌蛋白质合成时，这两条带消失。本研究清楚地表明，细菌蛋白质表达受与乳腺上皮细胞共培养的影响而受到调控。
