Keesee S K, Marchese J, Meneses A, Potz D, Garcia-Cuellar C, Szaro R P, Solorza G, Osornio-Vargas A, Mohar A, de la Garza J G, Wu Y J
Matritech, Inc., 330 Nevada Street, Newton, Massachusetts, 02160, USA.
Exp Cell Res. 1998 Oct 10;244(1):14-25. doi: 10.1006/excr.1998.4202.
The nuclear matrix is the nonchromatin protein structural component of the nucleus that governs nuclear shape and also exerts regulatory control over higher order gene organization. Recent studies have documented the presence of tumor-associated nuclear matrix proteins in several human cancers. We used high-resolution two-dimensional gel electrophoresis to compare nuclear matrix protein patterns in cervical carcinomas with those from normal cervical tissue. Tumors obtained from 20 patients undergoing hysterectomy for clinically localized cervical cancer were compared with normal cervical tissue. We have identified five polypeptides (CvC-1: Mr = 69,408 Da, pI = 5. 78; CvC-2: Mr = 53,752 Da, pI = 5.54; CvC-3: Mr = 47,887 Da, pI = 5. 60; CvC-4: Mr = 46,006 Da, pI = 5.07; and CvC-5: Mr = 44,864 Da, pI = 6.61) in the nuclear matrix from cervical carcinomas that were present in 20 of 20 cervical tumors but 0 of 10 normal tissues. These data extend similar findings of cancer-associated nuclear matrix proteins in other human cancers and suggest that nuclear matrix proteins may represent a new class of cancer markers that could aid the diagnosis or management of some types of cancer.
核基质是细胞核中一种非染色质蛋白结构成分，它决定细胞核的形状，并对高阶基因组织发挥调控作用。最近的研究已证明在几种人类癌症中存在肿瘤相关核基质蛋白。我们使用高分辨率二维凝胶电泳，比较宫颈癌与正常宫颈组织的核基质蛋白模式。将20例因临床局限性宫颈癌接受子宫切除术患者的肿瘤组织与正常宫颈组织进行比较。我们已鉴定出五种多肽（CvC - 1：相对分子质量 = 69,408 Da，等电点 = 5.78；CvC - 2：相对分子质量 = 53,752 Da，等电点 = 5.54；CvC - 3：相对分子质量 = 47,887 Da，等电点 = 5.60；CvC - 4：相对分子质量 = 46,006 Da，等电点 = 5.07；以及CvC - 5：相对分子质量 = 44,864 Da，等电点 = 6.61），这些多肽存在于20例宫颈癌的核基质中，而在10例正常组织中均未出现。这些数据扩展了在其他人类癌症中有关癌症相关核基质蛋白的类似发现，并表明核基质蛋白可能代表一类新的癌症标志物，有助于某些类型癌症的诊断或治疗。
