Arai S, Tsuji M, Kim S J, Nakade T, Kanno Y, Ishihara C
School of Veterinary Medicine, Rakuno-gakuen University, Ebetsu, Japan.
Int J Parasitol. 1998 Sep;28(9):1429-35. doi: 10.1016/s0020-7519(98)00094-0.
We have developed a mouse model for Babesia canis infection using severe combined immunodeficiency (SCID) mice whose circulating red blood cells had been substituted with canine red blood cells. Substitution of red blood cells in SCID mice was achieved by repetitive transfusions of canine red blood cells, together with administration of an antimouse red blood cell monoclonal antibody. Following inoculation of canine-red blood cell-SCID mice with B. canis, parasites proliferated in the canine red blood cells that had been transfused into the SCID mice, resulting in much higher parasitaemia than that observed in dogs. In an attempt to demonstrate the utility of this mouse model, three antiprotozoal drugs, diminazene diaceturate, clindamycin and oxytetracycline, were examined for their efficacy to inhibit the growth of B. canis in canine-red blood cell-SCID mice. The mouse model clearly showed that diminazene diaceturate and oxytetracycline were capable of eliminating B. canis from the canine-red blood cell-SCID mice, whereas clindamycin exhibited only a static effect as parasitaemia relapsed upon cessation of drug administration.
我们利用严重联合免疫缺陷（SCID）小鼠建立了犬巴贝斯虫感染的小鼠模型，这些小鼠的循环红细胞已被犬红细胞替代。通过重复输注犬红细胞并给予抗小鼠红细胞单克隆抗体，实现了SCID小鼠红细胞的替代。在用犬巴贝斯虫接种犬红细胞-SCID小鼠后，寄生虫在输注到SCID小鼠体内的犬红细胞中增殖，导致寄生虫血症比在犬中观察到的要高得多。为了证明该小鼠模型的实用性，研究了三种抗原生动物药物——双乙酰甲氧苄氨嘧啶、克林霉素和土霉素——对犬红细胞-SCID小鼠体内犬巴贝斯虫生长的抑制效果。该小鼠模型清楚地表明，双乙酰甲氧苄氨嘧啶和土霉素能够从犬红细胞-SCID小鼠体内清除犬巴贝斯虫，而克林霉素仅表现出抑制作用，因为停药后寄生虫血症会复发。
