Laboureau E, Vijayalakshmi M A
Laboratoire d'Interaction Moléculaire et de Technologie des Séparations, Université de Technologie de Compiègne, France.
J Mol Recognit. 1997 Nov-Dec;10(6):262-8. doi: 10.1002/(SICI)1099-1352(199711/12)10:6<262::AID-JMR377>3.0.CO;2-9.
The effect of introducing an immobilized metal ion ligand in the lower phase of the PEG/Dextran system was studied on the erythrocytes and lymphocytes partition. The ligand in the lower phase was added as an insoluble form [Sepharose-IDA-M(II)] with or without a ligand in the upper phase. We first checked that the addition of the insoluble ligand in the system did not affect the phase volume and settling, and also that Sepharose-IDA-M(II) partitioned strictly in the lower phase. Then we studied the partition of cells with various concentrations of ligand in the lower and upper phases. We clearly demonstrate here that the partition in immobilized metal ion affinity partitioning (IMAP) systems is correlated with the affinity between the cell surface and the ligand. Cells are attracted to the ligand-containing phase. This fact is important not only for the greater understanding of IMAP, but could also for the separation of some types of cells.
研究了在聚乙二醇/葡聚糖系统的下层相中引入固定化金属离子配体对红细胞和淋巴细胞分配的影响。下层相中的配体以不溶性形式[Sepharose-IDA-M(II)]添加，上层相中有或没有配体。我们首先检查了系统中添加不溶性配体不会影响相体积和沉降，并且Sepharose-IDA-M(II)严格分配在下层相中。然后我们研究了细胞在下层相和上层相中不同浓度配体存在下的分配情况。我们在此清楚地证明，固定化金属离子亲和分配（IMAP）系统中的分配与细胞表面和配体之间的亲和力相关。细胞被吸引到含配体的相中。这一事实不仅对于更深入理解IMAP很重要，而且对于某些类型细胞的分离也很重要。
