Fredén H, Linde A, Magnusson B C
Acta Odontol Scand. 1975;33(3):143-8. doi: 10.3109/00016357509026355.
The inorganic pyrophosphatase (PPiase) activity was determined by a colorimetric method in the odontoblasts and the parts of the enamel organ related to enamel matrix formation and enamel maturation. The effects on PPi hydrolysis by EDTA, R 8231, urea and heat treatment were found to be almost identical to those reported for nonspecific alkaline phosphatase (APase) in the same tissues. The Mg2+ activation curve for PPiase was also similar. Like those of APase, these characteristics of PPiase activity were identical in the three locations studied. It is suggested that the close similarity in the properties of PPiase and APase is due to activity of the same enzyme, a concept which is in agreement with recent biochemical and histochemical studies of calcification.
采用比色法测定成牙本质细胞以及牙釉质器官中与釉质基质形成和釉质成熟相关部分的无机焦磷酸酶（PPiase）活性。发现EDTA、R 8231、尿素和热处理对PPi水解的影响与在相同组织中报道的非特异性碱性磷酸酶（APase）的影响几乎相同。PPiase的Mg2+激活曲线也相似。与APase一样，PPiase活性的这些特征在所研究的三个部位是相同的。有人提出，PPiase和APase性质的密切相似性是由于同一种酶的活性，这一概念与最近关于钙化的生化和组织化学研究一致。
