Sun Y, Zhang W, Ma F, Chen W, Hou S
Center of Orthopedic Surgery, 304th Hospital of PLA, Beijing, China.
Chin Med J (Engl). 1997 Dec;110(12):927-31.
To evaluate the effect of transforming growth factor-beta/bone morphogenetic protein (TGF-beta/BMP) composite on the healing of large segmental bone defects and to discuss the interaction between TGF-beta and BMP during bone repair.
A 1.5 cm segmental defect was made in the mid-upper part of the radial shaft of 48 adult rabbits. The defects were filled with implants of TGF-beta/carrier, BMP/carrier or TGF-beta/BMP/carrier separately. 120 micrograms of purified bovine TGF-beta and 12 mg of BMP were used in the composite. The defects were examined radiographically and histologically at 4, 8, 12 and 16 weeks post-operation.
In the group filled with TGF-beta/BMP composite, the defect areas were bridged at 4 weeks, with callus of uniform radiodensity. Cortices of the cut ends were obscured by new bone. By 16 weeks post-operation, the defects were bridged by uniform new bone and the cut ends of the cortex could not be seen in all groups. In group of BMP/carrier, the defects were filled with more irregular woven bone callus than in other two groups. TGF-beta/BMP implanted defects in animals killed at 16 weeks showed histologically new lamella and woven bone that was formed in continuity with the cut ends of the cortex. Medullar canal was recanalized and contained marrow elements with normal appearance.
These data demonstrate not only the synergistic action between TGF-beta and BMP in the process of bone healing, but also a better effect of TGF-beta/BMP composite than single TGF-beta or BMP on bone repair, especially in the early stage of bone repair process.
评估转化生长因子-β/骨形态发生蛋白（TGF-β/BMP）复合物对大段骨缺损愈合的影响，并探讨骨修复过程中TGF-β与BMP之间的相互作用。
在48只成年兔桡骨中上段制造1.5 cm的节段性缺损。缺损分别用TGF-β/载体、BMP/载体或TGF-β/BMP/载体植入物填充。复合物中使用120微克纯化的牛TGF-β和12毫克BMP。术后4、8、12和16周对缺损进行影像学和组织学检查。
在填充TGF-β/BMP复合物的组中，缺损区域在4周时被骨痂桥接，骨痂放射密度均匀。断端皮质被新骨遮盖。术后16周时，所有组的缺损均被均匀的新骨桥接，皮质断端均不可见。在BMP/载体组中，缺损填充的不规则编织骨痂比其他两组更多。在16周处死的动物中，植入TGF-β/BMP的缺损在组织学上显示出新的板层骨和与皮质断端连续形成的编织骨。髓腔再通，含有外观正常的骨髓成分。
这些数据不仅证明了TGF-β与BMP在骨愈合过程中的协同作用，还表明TGF-β/BMP复合物在骨修复方面比单一的TGF-β或BMP效果更好，尤其是在骨修复过程的早期阶段。
