[Suppression of metastatic phenotype of cloned mouse lung adenocarcinoma cells by transfer of human genomic DNA].

OBJECTIVE

To isolate and identify human sequences with metastatic suppression ability.

METHODS

Genomic DNA fragments isolated from normal human lung tissue were transfected into cloned highly metastatic mouse lung adenocarcinoma cells together with PSV2neo as selectable marker.

RESULTS

25 transfectants were cloned in medium containing G418 and Ouabain. Eight morphologically flat revertants exhibited a more normal phenotype, six clones containing human DNA were identified by a sensitive Inter Alu-PCR method. The rate of cell growth and colony formation in agar were detected in vitro. Clone 12, 20 and 32 showed a lower ratio than maternal untransfected cells. In vivo clone 12 showed more significent less spontaneous metastases in nude mice and syngeneic T739 mouse than control group.

CONCLUSION

The results indicated that the inserted human DNA may be responsible for suppression of metastatic phenotype of mouse lung adenocarcinoma cells.