Song L, Bai S, Yang W
Institute for Environmental Surveillance, Chinese Academy of Preventive Medicine, Beijing.
Zhonghua Yi Xue Za Zhi. 1997 May;77(5):348-50.
To study the effect of transforming growth factor beta 1 on tumor cells.
HL-60 cells were transfected with transforming growth factor beta 1 expression vector by electroporation. Stable cell clones resistant to G 418 were obtained after 2-3 week growth with the drug-containing medium. A series of malignant phenotype were compared between transfected and untransfected HL 60 cells.
The growth speed of HL-60 cells which overexpressed transforming growth factor beta 1 mRNA decreased significantly. Clonigenecity in soft agarase and tumor formation in athymic mice were lower than those untransfected HL-60 cells.
Suppression effect of transforming growth factor beta 1 on malignant phenotype in HL-60 cells were demonstrated.
研究转化生长因子β1对肿瘤细胞的作用。
采用电穿孔法将转化生长因子β1表达载体转染人HL-60细胞。用含G418的培养基培养2-3周后，获得对G418耐药的稳定细胞克隆。比较转染和未转染的HL-60细胞之间的一系列恶性表型。
过表达转化生长因子β1 mRNA的HL-60细胞生长速度显著降低。其在软琼脂中的克隆形成能力及在裸鼠体内的成瘤能力均低于未转染的HL-60细胞。
证实了转化生长因子β1对HL-60细胞恶性表型具有抑制作用。
