[从中国急性散发性戊型肝炎患者血清中分离出的7株戊型肝炎病毒的部分核苷酸序列分析]

[Partial nucleotide sequencing of 7 hepatitis E virus strains isolated from sera of patients with acute sporadic hepatitis E in China].

作者信息

Zhu X, Zhuang H, Zhu W

机构信息

Department of Microbiology, Beijing Medical University, Beijing.

出版信息

Zhonghua Yi Xue Za Zhi. 1997 Jun;77(6):447-9.

PMID:9772511

Abstract

OBJECTIVE

To compare partial nucleotide and amino acid sequences between epidemic and sporadic hepatitis E viruses (HEV) in China and their relation to the frequency of hepatitis E (HE).

METHODS

A reverse transcription nested polymerase chain reaction (RT-nPCR) was used for the amplication of HEV open reading frame 2 (ORF2) cDNA from sera of patients with acute sporadic HE. The RT-nPCR products of HEV ORF2 cDNA were directly sequenced using a sequenase version 2.0 kit with the dideoxy sequencing method.

RESULTS

28 of 41 sera of patients with acute sporadic HE obtained from Shenzhen, Changchun, Hangzhou, Xian and Beijing by RT-nPCR were HEV ORF2 cDNA positive, and 7 of them were sequenced. The nucleotide homology of the seven HEV ORF2 cDNA was 78.9%-80.2%, as compared with the Mexico strain of HEV (M); 93.1%-95.1% with the Burma epidemic strain of HEV (B) (Ep); 92.3%-94.2% with the Burma sporadic strain of HEV (B) (Sp); 96.5%-98.9% with the Xinjiang epidemic strain of HEV CH 1.1; and 95.7%-100.0% between them.

CONCLUSIONS

The seven strains of HEV ORF2 cDNA isolated from sera of acute sporadic HE cases have a higher degree of homology with the HEV CH 1.1 and the HEV (B), and may belong to HEV (B) subtype, as the HEV CH1.1 does.

摘要

目的

比较中国流行株和散发株戊型肝炎病毒（HEV）的部分核苷酸和氨基酸序列，及其与戊型肝炎（HE）发病频率的关系。

方法

采用逆转录巢式聚合酶链反应（RT-nPCR）从急性散发性戊型肝炎患者血清中扩增HEV开放阅读框2（ORF2）cDNA。使用测序酶2.0试剂盒和双脱氧测序法对HEV ORF2 cDNA的RT-nPCR产物进行直接测序。

结果

通过RT-nPCR从深圳、长春、杭州、西安和北京收集的41例急性散发性戊型肝炎患者血清中，28例HEV ORF2 cDNA呈阳性，其中7例进行了测序。这7株HEV ORF2 cDNA与墨西哥株HEV（M）的核苷酸同源性为78.9%-80.2%；与缅甸流行株HEV（B）（Ep）为93.1%-95.1%；与缅甸散发株HEV（B）（Sp）为92.3%-94.2%；与新疆流行株HEV CH 1.1为96.5%-98.9%；它们之间为95.7%-100.0%。