Zhu X, Zhuang H, Zhu W
Department of Microbiology, Beijing Medical University.
Zhonghua Yu Fang Yi Xue Za Zhi. 1996 Nov;30(6):334-6.
A reverse transcription-nested polymerase chain reaction (RT-nPCR) for detection of hepatitis E virus (HEV) RNA in serum was established using two pairs of oligonucleotide acid from open reading frame 1 and 2 (ORF1 and ORF2) of HEV cDNA as the inner and outer primers. Eleven serial sera from two rhesus monkeys experimentally infected with HEV were tested for HEV RNA with established RT-nPCR. HEV RNA became detectable in these two monkeys on the sixth day and the ninth day, respectively, and persisted up to the 17th and the 22nd day after infection, respectively. Twenty-eight serum specimens of the 41 patients with acute sporadic hepatitis E were HEV RNA positive (68.3%). Proportions of positive HEV RNA in patients sera collected one to ten days, 11 to 20 days and 21 to 34 days after their onset of the disease were 72.7% (16/22), 70.0% (7/10) and 55.5% (5/9), respectively. It suggests that viremia of HEV lasts a shorter period, and proportion of serum positive HEV RNA decreases with the course of the disease.
采用来自戊型肝炎病毒（HEV）cDNA开放阅读框1和2（ORF1和ORF2）的两对寡核苷酸作为内引物和外引物，建立了用于检测血清中HEV RNA的逆转录巢式聚合酶链反应（RT-nPCR）。用建立的RT-nPCR对两只经实验感染HEV的恒河猴的11份连续血清进行HEV RNA检测。在这两只猴子中，分别在感染后的第6天和第9天检测到HEV RNA，并且分别持续到感染后的第17天和第22天。41例急性散发性戊型肝炎患者的28份血清标本HEV RNA呈阳性（68.3%）。在发病后1至10天、11至20天和21至34天采集的患者血清中，HEV RNA阳性比例分别为72.7%（16/22）、70.0%（7/10）和55.5%（5/9）。这表明HEV病毒血症持续时间较短，并且血清HEV RNA阳性比例随病程而降低。
