[尿激酶型纤溶酶原激活剂在天疱疮发病机制中的作用]
[The role of urokinase-type plasminogen activator in the pathogenesis of pemphigus].
作者信息
Nie Z, Liu R, Ye Q
机构信息
Department of Dermatology, Southwest Hospital Third Military Medical University, Chongquing.
出版信息
Zhonghua Yi Xue Za Zhi. 1997 Oct;77(10):771-3.
OBJECTIVE
To investigate the changes of urokinase-type plasmino-gen activator (u-PA) in the process of acantholysis in pemphigus and observe the influence of purified urokinase on the cultured skin explant.
METHODS
The expression of urokinase antigen on the pemphigus organ model was revealed by immunohistochemical method; PA activity was assayed in the acantholysis model; the influence of urokinase on the epidermis was observed by adding purified urokinase into the cultured skin explant.
RESULTS
PA activity was elevated in the acantholysis model at 24 hour and was continuing its increase at 48 and 72 hours. The expression of urokinase was high in the epidermis of pemphigus organ model; purified urokinase could induce acantholysis like changes.
CONCLUSION
Pemphigus antibody induces acantholysis through activating keratinocytes. The latter secretes an elevated u-PA, which locates on the membrane of the epidermal cells producing a limited pro-teolysis which damages the cohesion of epidermal cells.
目的
研究天疱疮棘层松解过程中尿激酶型纤溶酶原激活剂（u-PA）的变化，并观察纯化尿激酶对培养皮肤外植体的影响。
方法
采用免疫组化方法检测天疱疮器官模型中尿激酶抗原的表达；在棘层松解模型中测定纤溶酶原激活剂（PA）活性；将纯化尿激酶加入培养的皮肤外植体中，观察尿激酶对表皮的影响。
结果
棘层松解模型在24小时时PA活性升高，48小时和72小时时持续升高。天疱疮器官模型表皮中尿激酶表达较高；纯化尿激酶可诱导类似棘层松解的变化。
结论
天疱疮抗体通过激活角质形成细胞诱导棘层松解。后者分泌升高的u-PA，其定位于表皮细胞膜上，产生有限的蛋白水解作用，破坏表皮细胞的黏附力。
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