Wilkinson J E, Smith C A, Suter M M, Falchek W, Lewis R M
Department of Pathobiology, University of Tennessee, Knoxville 37901.
Am J Pathol. 1989 Mar;134(3):561-9.
The role of plasminogen activator (PA) in the pathogenesis of acantholysis in canine pemphigus vulgaris (PV) was evaluated using differentiated cultures of canine oral keratinocytes. Both the secreted and cell-associated PA activity in cultured canine keratinocytes were completely inhibited by specific anti-urokinase antibodies. Anti-tissue type PA antibodies did not inhibit either secreted or cell-associated PA activity. Immunoblots and fibrin zymography revealed a single 57,000 molecular weight urokinase-type PA in the conditioned media of the canine oral keratinocytes. Incubation of the differentiated cultures with PV Ig resulted in a significant increased in the levels of PA activity and both canine and human PV Ig were effective at inducing acantholysis typical of that seen in the clinical disease. The addition of urokinase inhibitor to the cultures treated with PV Ig prevented the development of acantholysis. These data strongly support the conclusion that PA is involved in acantholysis which is the cardinal feature of PV.
利用犬口腔角质形成细胞的分化培养物，评估了纤溶酶原激活剂（PA）在犬寻常性天疱疮（PV）棘层松解发病机制中的作用。培养的犬角质形成细胞中分泌型和细胞相关的PA活性均被特异性抗尿激酶抗体完全抑制。抗组织型PA抗体既不抑制分泌型PA活性，也不抑制细胞相关的PA活性。免疫印迹和纤维蛋白酶谱分析显示，犬口腔角质形成细胞条件培养基中有一条分子量为57,000的单一尿激酶型PA条带。用PV Ig孵育分化培养物导致PA活性水平显著升高，犬和人PV Ig均能有效诱导出临床疾病中典型的棘层松解。向用PV Ig处理的培养物中添加尿激酶抑制剂可防止棘层松解的发生。这些数据有力地支持了PA参与棘层松解这一结论，而棘层松解是PV的主要特征。
