单克隆抗体7E12H12可区分膀胱原发性腺癌和前列腺原发性腺癌。
The monoclonal antibody 7E12H12 can differentiate primary adenocarcinoma of the bladder and prostate.
作者信息
Pantuck A J, Murphy D P, Amenta P S, Das K M, Cummings K B, Weiss R E
机构信息
Division of Urology, Robert Wood Johnson Medical School, New Brunswick, NJ, USA.
出版信息
Br J Urol. 1998 Sep;82(3):426-30. doi: 10.1046/j.1464-410x.1998.00755.x.
OBJECTIVE
To determine if the monoclonal antibody 7E12H12, which reacts with a 40 kDa protein in normal human enterocytes and has been shown to be a marker for intestinal metaplasia and adenocarcinoma arising in the bladder, could assist in distinguishing prostatic, urachal and vesical adenocarcinoma, using a sensitive immunohistochemical assay.
MATERIALS AND METHODS
Fifteen primary prostatic adenocarcinomas and five adenocarcinomas of the urinary bladder were selected for a retrospective evaluation. The monoclonal antibody 7E12H12 (IgM isotype) was used in an immunoperoxidase assay to survey formalin-fixed, paraffin-embedded archival tissue specimens.
RESULTS
All vesical adenocarcinomas reacted positively with the antibody, regardless of grade; none of the 15 prostatic specimens reacted positively in either the benign or malignant glandular epithelium.
CONCLUSION
The monoclonal antibody 7E12H12 can differentiate primary adenocarcinoma of the bladder from secondary adenocarcinoma arising in the prostate and may be a useful tool in diagnostic pathology.
目的
使用灵敏的免疫组织化学检测法，确定单克隆抗体7E12H12能否有助于鉴别前列腺癌、脐尿管癌和膀胱腺癌。该抗体可与正常人肠上皮细胞中的一种40 kDa蛋白发生反应，已被证明是膀胱肠化生和腺癌的标志物。
材料与方法
选取15例原发性前列腺癌和5例膀胱腺癌进行回顾性评估。采用免疫过氧化物酶检测法，使用单克隆抗体7E12H12（IgM同种型）检测福尔马林固定、石蜡包埋的存档组织标本。
结果
所有膀胱腺癌，无论分级如何，均与该抗体呈阳性反应；15例前列腺标本中，无论是良性还是恶性腺上皮均无阳性反应。
结论
单克隆抗体7E12H12可将原发性膀胱癌与前列腺来源的继发性腺癌区分开来，可能是诊断病理学中的一种有用工具。
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