Hsieh H M, Huang P C
Department of Nursing, Yuanpei Institute of Medical Technology, Taiwan, ROC.
DNA Seq. 1998 Mar;9(1):9-17. doi: 10.3109/10425179809050020.
A novel stress-inducible metallothionein-like gene from rice, designated as rgMT-1 (rice genomic metallothionein-like gene-1), was isolated and sequenced. From the sequence analysis of its 5'-flanking region, two putative TATA boxes, one CAAT box, and several short sequences homologous to regulatory cis-elements previously reported were identified. Two direct repeats, one 10 bp in length (CAAAATCAAA) and the other 11 bp (GTGAAAATACT), respectively, were also found. By transient GUS (beta-glucuronidase) assay, the expression of GUS, in vitro, was enhanced by the presence of the rgMT-1 intron. The critical region which controls the basal transcription was shown to lie between -73 and -36 upstream of rgMT-1, in which one of the two putative TATA boxes was located. The promoter activity was lost completely when both putative TATA boxes were deleted. This is the first report describing the genomic structure and regulation of a monocotyledonous metallothionein-like gene critical to the response of stress.
从水稻中分离并测序了一个新的应激诱导型类金属硫蛋白基因，命名为rgMT-1（水稻基因组类金属硫蛋白基因-1）。通过对其5'侧翼区的序列分析，鉴定出两个推定的TATA盒、一个CAAT盒以及几个与先前报道的调控顺式元件同源的短序列。还发现了两个直接重复序列，一个长度为10 bp（CAAAATCAAA），另一个为11 bp（GTGAAAATACT）。通过瞬时GUS（β-葡萄糖醛酸酶）检测，rgMT-1内含子的存在增强了体外GUS的表达。控制基础转录的关键区域位于rgMT-1上游-73至-36之间，其中两个推定的TATA盒之一位于该区域。当两个推定的TATA盒都缺失时，启动子活性完全丧失。这是第一份描述单子叶植物类金属硫蛋白基因的基因组结构及其对应激反应至关重要的调控的报告。
