Weimann E, Rutkowski S, Reisbach G
University of Frankfurt, Medical Centre for Child Health, Germany.
J Perinat Med. 1998;26(3):211-8. doi: 10.1515/jpme.1998.26.3.211.
The objectives of this study were 1) to clarify the physiologic regulation of cytokines such as IL-6, G-CSF and GM-CSF in preterm and term neonates and 2) to evaluate the influence of perinatal stress and infection on endogenous cytokine levels.
We examined cord blood levels of G-CSF, GM-CSF and IL-6 using a bioassay in 43 term and 44 preterm neonates.
Compared to normal neonates (G-CSF: mean (m) = 97.6 +/- 16.3 pg/ml; IL-6: m = 20.2 +/- 4.6 pg/ml), we found elevated G-CSF levels in newborns with perinatal stress (m = 247.1 +/- 72.1 pg/ml; p = 0.003) and increased levels for G-CSF (m = 8980.9 +/- 4388 pg/ml; p = 0.0003) and IL-6 (m = 705 +/- 322.3 pg/ml; p = 0.025) in neonates with infection. Term newborns with infection had higher G-CSF levels than preterms (m = 15575 +/- 9374 pg/ml versus m = 5384.1 +/- 4470.9 pg/ml; p = 0.024). G-CSF levels of newborns with infection were correlated with birth weight (r = 0.50; p = 0.024) but not with gestational age (r = 0.40; p = 0.057). GM-CSF was only detectable in cord blood in 4 cases of normal healthy neonates.
The response of G-CSF levels in preterms to infection is diminished. Body cell mass is more important than gestational age to provide high G-CSF levels during states of infection.
本研究的目的是1）阐明早产和足月新生儿中白细胞介素-6（IL-6）、粒细胞集落刺激因子（G-CSF）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）等细胞因子的生理调节，以及2）评估围产期应激和感染对内源性细胞因子水平的影响。
我们采用生物测定法检测了43例足月新生儿和44例早产新生儿脐带血中G-CSF、GM-CSF和IL-6的水平。
与正常新生儿相比（G-CSF：均值（m）=97.6±16.3 pg/ml；IL-6：m=20. /span>2±4.6 pg/ml），我们发现围产期应激新生儿的G-CSF水平升高（m=247.1±72.1 pg/ml；p=0./span>003），感染新生儿的G-CSF（m=8980.9±4388 pg/ml；p=0./span>0003）和IL-6（m=705±322.3 pg/ml；p=0./span>025）水平升高。感染的足月新生儿的G-CSF水平高于早产新生儿（m=15575±9374 pg/ml对m=5384.1±4470.9 pg/ml；p=0./span>024）。感染新生儿的G-CSF水平与出生体重相关（r=0.50；p=0./span>024），但与胎龄无关（r=0.40；p=0./span>057）。GM-CSF仅在4例正常健康新生儿的脐带血中可检测到。
早产儿对感染时G-CSF水平的反应减弱。在感染状态下，身体细胞质量比胎龄更重要，以提供高水平的G-CSF。
