避孕套失效的客观指标。
Objective markers of condom failure.
作者信息
Lawson M L, Maculuso M, Bloom A, Hortin G, Hammond K R, Blackwell R
机构信息
Department of Epidemiology, School of Public Health, University of Alabama at Birmingham 35294-2170, USA.
出版信息
Sex Transm Dis. 1998 Sep;25(8):427-32. doi: 10.1097/00007435-199809000-00009.
BACKGROUND
Studies of condom efficacy rely on self-reported behavior. Objective markers of exposure to semen may provide a more valid assessment of condom failure and failure to use condoms.
GOALS OF THIS STUDY
To compare three semen biomarkers: acid phosphatase (AP) activity, prostate specific antigen (PSA), and the human seminal plasma antigen (MHS-5).
STUDY DESIGN
Twenty women were intravaginally inoculated with six measured, increasingly larger amounts of their partners' semen. Vaginal fluid was collected by the participant using swabs and tested.
RESULTS
Background levels of PSA were low (0.00-1.25 ng/ml), background levels of AP were variable (0-350 U/l), and all preinoculation samples were negative for MHS-5. All postinoculation samples were positive for PSA, 64 of 117 (55%) for AP, and 14 of 120 (12%) for MHS-5.
CONCLUSION
The PSA immunoassay was the best semen biomarker under these sampling and testing conditions.
背景
避孕套有效性的研究依赖于自我报告行为。精液接触的客观标志物可能为避孕套失败和未使用避孕套提供更有效的评估。
本研究的目的
比较三种精液生物标志物：酸性磷酸酶（AP）活性、前列腺特异性抗原（PSA）和人精浆抗原（MHS-5）。
研究设计
20名女性经阴道接种了六次测量的、量逐渐增加的其伴侣的精液。参与者使用拭子收集阴道液并进行检测。
结果
PSA的背景水平较低（0.00 - 1.25 ng/ml），AP的背景水平各不相同（0 - 350 U/l），且所有接种前样本的MHS-5均为阴性。所有接种后样本的PSA均为阳性，117份样本中有64份（55%）的AP为阳性，120份样本中有14份（12%）的MHS-5为阳性。
结论
在这些采样和检测条件下，PSA免疫测定是最佳的精液生物标志物。
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