Romain N, Gesell M S, Leroy O, Forget P, Dandrifosse G, Luk G D
Department of Biochemistry and General Physiology, University of Liege, Belgium.
Comp Biochem Physiol A Mol Integr Physiol. 1998 Jun;120(2):379-84. doi: 10.1016/s1095-6433(98)10040-5.
The effect of oral administration of spermine on pancreatic maturation was investigated in the suckling rat. The treatment consisted of 0.3-0.4 mmol spermine kg-1 body weight given orally once a day for 3 days starting at day 11 after birth. Spermine administration does not adversely affect the growth of the pancreas (wet weight, protein and DNA contents remain unchanged). The proliferating cell nuclear antigen (PCNA) index decreases significantly in spermine-treated rats, indicating that spermine slows down the proliferation rate of the organ. The enzymatic activities of trypsin, chymotrypsin and alpha-amylase are increased significantly in the pancreas of spermine-treated rats. The morphology of the organ seems affected as shown by hematoxylin-eosin staining: a cytoplasm indicative of higher synthetic activity is visible after spermine treatment. We conclude that spermine treatment of unweaned rats can induce precocious biochemical and morphological maturation of the exocrine pancreas, pushing the organ forward in the process of differentiation (closer to the adult stage).
研究了口服精胺对乳鼠胰腺成熟的影响。治疗方法为从出生后第11天开始，每天口服一次0.3 - 0.4 mmol精胺/千克体重，持续3天。给予精胺对胰腺生长没有不利影响（湿重、蛋白质和DNA含量保持不变）。在经精胺处理的大鼠中，增殖细胞核抗原（PCNA）指数显著降低，表明精胺减缓了该器官的增殖速率。在经精胺处理的大鼠胰腺中，胰蛋白酶、糜蛋白酶和α-淀粉酶的酶活性显著增加。苏木精-伊红染色显示该器官的形态似乎受到影响：精胺处理后可见具有较高合成活性的细胞质。我们得出结论，对未断奶大鼠进行精胺处理可诱导外分泌胰腺早熟的生化和形态成熟，使该器官在分化过程中向前推进（更接近成年阶段）。
