Trachtenberg S
Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School, Jerusalem, 91120, Israel.
J Struct Biol. 1998 Sep;123(1):45-55. doi: 10.1006/jsbi.1998.4015.
The design and construction of a fast-freezing device are described. A polycarbonate chamber, in which the humidity and temperature are controlled by microprocessors, slides on a robust chassis guided by ball or Teflon bushings. In its freezing position, the chamber rests on top of the cryogen vessel. The specimen is therefore frozen directly from the experimental conditions within the chamber without exposure to the external environment. After freezing, the chamber, but not the specimen, rises automatically, vacating space for handling the specimen. The chamber, the shutter, and the specimen are all driven pneumatically at an adjustable speed of up to approximately 10 m s-1 and coordinated by either pneumatic or electronic logical gates. Provisions are made for automatic blotting, spraying, and flashing. The chamber is compatible with a liquid nitrogen-cooled copper block assembly for impact (slam) freezing for freeze-substitution and freeze-fracture.
描述了一种快速冷冻装置的设计与构造。一个聚碳酸酯腔室，其湿度和温度由微处理器控制，在由滚珠或聚四氟乙烯衬套引导的坚固底盘上滑动。在其冷冻位置，腔室放置在低温容器顶部。因此，样本直接在腔室内的实验条件下被冷冻，而不会暴露于外部环境。冷冻后，腔室（而非样本）自动升起，为处理样本腾出空间。腔室、快门和样本均由气动驱动，速度可调，最高可达约10米/秒，并由气动或电子逻辑门协调。具备自动吸干、喷雾和闪蒸功能。该腔室与液氮冷却的铜块组件兼容，用于冲击（猛击）冷冻以进行冷冻置换和冷冻断裂。
