Wechselberger C
Institute of Molecular Biology, Austrian Academy of Sciences, Department of Biochemistry, Billrothstr. 11, 5020-Salzburg, Austria.
Biochim Biophys Acta. 1998 Oct 14;1388(1):279-83. doi: 10.1016/s0167-4838(98)00202-7.
Dermaseptins are a group of basic (lysine-rich) peptides, 27-34 amino acids in length and involved in the defense of frog skin against microbial invasion. By using a degenerated oligonucleotide primer binding to the 5'-untranslated region of previously characterized cDNAs of these peptides, it was possible to identify new members of the dermaseptin family in the South American frogs Agalychnis annae and Pachymedusa dacnicolor. Amino acid alignment and secondary structure prediction reveals, that only five of the deduced peptides can be supposed to be also functional homologs to the known dermaseptins from Phyllomedusa bicolor and Phyllomedusa sauvagei. The remaining six peptides described in this paper have not been isolated and characterized yet.
皮肤防御素是一组碱性（富含赖氨酸）肽，长度为27 - 34个氨基酸，参与青蛙皮肤抵御微生物入侵的防御过程。通过使用与这些肽先前已鉴定的cDNA的5'非翻译区结合的简并寡核苷酸引物，有可能在南美青蛙安娜红眼树蛙（Agalychnis annae）和达氏壮发蛙（Pachymedusa dacnicolor）中鉴定出皮肤防御素家族的新成员。氨基酸序列比对和二级结构预测表明，推测出的肽中只有五个可能也是双色叶泡蛙（Phyllomedusa bicolor）和索氏叶泡蛙（Phyllomedusa sauvagei）中已知皮肤防御素的功能同源物。本文描述的其余六个肽尚未分离和鉴定。
