用于检测破伤风抗毒素的乳胶凝集试验。
Latex agglutination test for detection of tetanus antitoxins.
作者信息
Lee C L, Huang H C, Chiu S Y, Lee Y S, Pan T M
机构信息
National Institute of Preventive Medicine, Department of Health, Taipei, Taiwan, R.O.C.
出版信息
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1995 May;28(2):151-6.
A rapid and easy method of slide agglutination test for the detection of human tetanus antitoxins was developed in this study. Testing reagents were prepared from carboxylated polystyrene latex particles with tetanus toxin by soluble carbodiimide. The test was performed on a glass slide with a drop of test sample and a drop of testing reagent. The agglutination reaction was usually completed within five minutes. Sensitivity of this test for tetanus antitoxins can be reached at 0.125 IU/ml. Therefore, the latex agglutination test can be used to determine the immune status of a patient in an emergency.
本研究开发了一种快速简便的玻片凝集试验方法，用于检测人破伤风抗毒素。检测试剂由羧化聚苯乙烯乳胶颗粒与破伤风毒素通过水溶性碳二亚胺制备而成。试验在玻片上进行，分别滴加一滴测试样品和一滴检测试剂。凝集反应通常在五分钟内完成。该试验对破伤风抗毒素的灵敏度可达0.125 IU/ml。因此，乳胶凝集试验可用于在紧急情况下确定患者的免疫状态。
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