葡萄球菌α毒素的蛋白水解降解

Proteolytic degradation of staphylococcal alpha-toxin.

作者信息

Dalen A B

出版信息

Acta Pathol Microbiol Scand B. 1976 Oct;84B(5):309-14. doi: 10.1111/j.1699-0463.1976.tb01943.x.

DOI:10.1111/j.1699-0463.1976.tb01943.x

Abstract

Staphylococcal alpha-toxin of mol.wt. 39,000 was degraded at an alkaline pH by staphylococcal extracellular proteases resulting in the formation of three relatively stable intermediates with mol.wt. 27,500, 23,500 and 12,000. The intermediate with mol.wt. 27,500 which existed in two charged forms, was isolated by column chromatography and found to be non-haemolytic. Furthermore, it could be obtained by proteolysis of alpha-toxin (mol.wt. 39,000) with chymotrypsin in low concentrations. This intermediate was further degraded by trypsin to the protein with mol.wt. 23,500 and 12,000.

摘要

分子量为39,000的葡萄球菌α毒素在碱性pH条件下被葡萄球菌细胞外蛋白酶降解，产生了三种分子量分别为27,500、23,500和12,000的相对稳定的中间体。分子量为27,500的中间体以两种带电形式存在，通过柱色谱法分离得到，发现其无溶血活性。此外，低浓度的胰凝乳蛋白酶对α毒素（分子量39,000）进行蛋白水解也可得到该中间体。该中间体再经胰蛋白酶进一步降解，产生分子量为23,500和12,000的蛋白质。