副溶血性弧菌锰抗性突变体的特性分析
Characterization of the manganese-resistant mutants derived from Vibrio parahaemolyticus.
作者信息
Weng J H, Wong H C, Liu C C, Lee S Y, Yu C M, Lee Y S, Tseng H C, Lu K Y
机构信息
Department of Microbiology, Soochow University, Taipei, Taiwan, R.O.C.
出版信息
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1995 Nov;28(4):256-69.
The virulence and some related factors of Vibrio parahaemolyticus are regulated by the level of iron. In this study, five Mn-resistant mutants were selected after N-methyl-N'-nitrosoguanidine treatment and two transfers in medium containing high levels of manganese chloride. Production of siderophores and the 77-kDa iron-regulated outer-membrane protein and the bacterial growth in these Mn-resistants were deregulated, as compared with the wild-type strain. In addition, the regulation of these phenomena was partially or completely restored by the introduction of Escherichia coli fur gene. Also, the total cellular protein profiles of the wild-type and mutants showed that production of some proteins were positively or negatively regulated by iron, and expression of some of these proteins remained unaffected in these mutants. These results suggested the presence of a complicated iron regulation system, similar to the Fur system of E. coli, in this pathogen.
副溶血性弧菌的毒力及一些相关因子受铁水平的调控。在本研究中，经N-甲基-N'-亚硝基胍处理并在含有高浓度氯化锰的培养基中传代两次后，筛选出了五个耐锰突变体。与野生型菌株相比，这些耐锰突变体中铁载体、77-kDa铁调节外膜蛋白的产生以及细菌生长均不受调控。此外，通过导入大肠杆菌fur基因，这些现象的调控得以部分或完全恢复。而且，野生型和突变体的总细胞蛋白谱显示，一些蛋白质的产生受铁的正调控或负调控，其中一些蛋白质的表达在这些突变体中不受影响。这些结果表明，在这种病原体中存在一个类似于大肠杆菌Fur系统的复杂铁调节系统。
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