Yang S F, Cheng J C, Hor L I
Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, R.O.C.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1995 Nov;28(4):270-9.
Restriction fragment length polymorphism analysis of rRNA genes (ribotyping) was used to differentiate Vibrio vulnificus isolates. Among the 10 restriction enzymes tested, HindIII was shown to provide the most discriminatory patterns. Stul was used for further analysis of strains that were indistinguishable with HindIII. Thirteen clinical V. vulnificus strains were analyzed for their ribotypes with HindIII, as well as Stul when necessary. Four of the clinical strains were isolated from different samples collected from the same patient, and were shown to have identical ribotypes. All the others gave unique ribotypes, indicating the large genetic divergence in V. vulnificus clinical isolates. The ribotype of V. vulnificus by HindIII remained unchanged after successive in vitro and in vivo passages. HindIII gave rise to five bands which were shown in every V. vulnificus strain but not in other vibrio species tested, suggesting that ribotyping with this restriction enzyme may be useful for confirming the identification of this bacterium.
利用rRNA基因的限制性片段长度多态性分析（核糖体分型）来区分创伤弧菌分离株。在所测试的10种限制性内切酶中，HindIII显示出提供最具鉴别性的图谱。当用HindIII无法区分菌株时，使用Stul进行进一步分析。对13株临床创伤弧菌菌株进行了HindIII核糖体分型分析，必要时也进行了Stul核糖体分型分析。其中4株临床菌株是从同一患者采集的不同样本中分离出来的，结果显示它们具有相同的核糖体分型。所有其他菌株都呈现出独特的核糖体分型，表明创伤弧菌临床分离株存在很大的遗传差异。创伤弧菌经连续的体外和体内传代后，其HindIII核糖体分型保持不变。HindIII产生了五条带，每条创伤弧菌菌株中都有这五条带，但在所测试的其他弧菌属物种中没有，这表明用这种限制性内切酶进行核糖体分型可能有助于确认该细菌的鉴定。
