Old J M, Deane E M
School of Science, University of Western Sydney, Nepean, NSW, Australia.
Comp Immunol Microbiol Infect Dis. 1998 Oct;21(4):237-45. doi: 10.1016/s0147-9571(98)00022-8.
Qualitative changes in the culturable, aerobic bacterial flora isolated from the tammar pouch have been documented over the period leading up to oestrus, at the time of anticipated birth and in absence and presence of pouch young of varying ages. In a group of 12 animals studied thirty species of aerobic bacteria were isolated. Twenty five species were found in pouches with no pouch young, 9 in pouches with young less than 3 weeks of age and 9 in pouches containing older animals. Gram positive organisms including Corynebacterium spp., Micrococcus spp. and Staphylococcus spp. were the most frequent isolates, regardless of reproductive status. Whilst Gram-negative rods Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter aerogenes, and Escherichia coli were found in pouches with and without pouch young, but not in pouches close to oestrus, at the time of birth or in the presence of very young animals (< 6 days). While pouches without pouch young displayed the greatest diversity of bacterial species, there were still significant numbers of bacterial species in those containing pouch young. Results suggest that the microbial population of the tammar pouch does not seriously compromise the well-being of the young animal during crucial stages of immunological development. The data are consistent with observations on the microflora of the quokka pouch but not with data from the koala.
在接近发情期、预期分娩时以及有无不同年龄育儿袋幼崽的情况下，对从袋鼠育儿袋中分离出的可培养需氧菌菌群的定性变化进行了记录。在一组12只动物中，共分离出30种需氧菌。在没有育儿袋幼崽的育儿袋中发现了25种，在有小于3周龄幼崽的育儿袋中发现了9种，在有较大幼崽的育儿袋中也发现了9种。无论生殖状态如何，革兰氏阳性菌，包括棒状杆菌属、微球菌属和葡萄球菌属，都是最常见的分离菌。虽然在有和没有育儿袋幼崽的育儿袋中都发现了革兰氏阴性杆菌，如肺炎克雷伯菌、铜绿假单胞菌、产气肠杆菌和大肠杆菌，但在接近发情期、分娩时或有非常年幼的动物（<6天）时，这些菌在育儿袋中未被发现。虽然没有育儿袋幼崽的育儿袋中细菌种类最多，但有育儿袋幼崽的育儿袋中也有大量细菌种类。结果表明，在免疫发育的关键阶段，袋鼠育儿袋中的微生物群不会严重损害幼崽的健康。这些数据与短尾矮袋鼠育儿袋微生物群的观察结果一致，但与树袋熊的数据不一致。
