Enaida H, Ito T, Oshima Y, Sakamoto T, Yago K, Kato K, Kochi H
Department of Biochemistry, Fukushima Medical University School of Medicine, Japan.
Fukushima J Med Sci. 1998 Jun;44(1):43-52.
We isolated microvascular endothelial cells from bovine retinas (BREC) by a colony isolation method, and investigated the effects of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) on growth as well as integrin subtype expression of the cultured BREC. Either of the growth factors stimulated growth of the cultured BREC. In non-stimulated BREC, alpha v beta 3 integrin expression was predominant over the other integrin subtypes tested (alpha v beta 5, alpha 2 beta 1 and alpha 5 beta 1), but no enhancement of the alpha v beta 3 expression occurred by either growth factor stimulation. On the other hand, bFGF addition stimulated alpha v beta 5 and alpha 5 beta 1 expression while VEGF induced the alpha 2 beta 1 expression as well. These results suggest that external stimuli such as bFGF and VEGF alter integrin expression profiles of BREC and probably influence interactions of endothelial cells with extracellular matrix during angiogenesis which must be involved in pathogenesis of ischemic retinal disorders. In this respect, this BREC culture system could be useful to study the mechanism of neovascularization in these disorders.
我们采用集落分离法从牛视网膜中分离出微血管内皮细胞（BREC），并研究了碱性成纤维细胞生长因子（bFGF）和血管内皮生长因子（VEGF）对培养的BREC生长以及整合素亚型表达的影响。两种生长因子均刺激了培养的BREC的生长。在未受刺激的BREC中，αvβ3整合素的表达高于其他所检测的整合素亚型（αvβ5、α2β1和α5β1），但两种生长因子刺激均未增强αvβ3的表达。另一方面，添加bFGF刺激了αvβ5和α5β1的表达，而VEGF也诱导了α2β1的表达。这些结果表明，诸如bFGF和VEGF等外部刺激会改变BREC的整合素表达谱，并可能在血管生成过程中影响内皮细胞与细胞外基质的相互作用，而这必定参与了缺血性视网膜疾病的发病机制。在这方面，这种BREC培养系统可能有助于研究这些疾病中的新生血管形成机制。
