表皮透明角质颗粒中的硫：通过X射线微量分析进行定量测定。

Sulphure in epidermal keratohyalin granules: a quantitative assay by x-ray microanalysis.

作者信息

Jessen H, Peters P D, Hall T A

出版信息

J Cell Sci. 1976 Oct;22(1):161-71. doi: 10.1242/jcs.22.1.161.

DOI:10.1242/jcs.22.1.161

Abstract

The elemental composition of different types of keratohyalin granules from the epidermis of newborn and adult rats was studied by means of an EMMA-4 analytical electron microscope, equipped with an energy-dispersive X-ray spectrometer. An absolute quantitation of the sulphur concentration in keratohyalin granules was performed. The results demonstrate that epidermal keratohyalin granules are chemically heterogeneous. A type of keratohyalin granule present in the nuclei and cytoplasm of epidermal cells from both newborn and adult rats - termed single granules - is rich in sulphur, having a content of 2-5-3-6%. Other types of keratohyalin granules, which differ in newborn and adult rats, contain a sulphur-poor component; they often have a sulphur-rich component as well. The sulphur-poor keratohyalin contains 0-6-0-9% sulphur. It is suggested that the sulphur-rich keratohyalin granules are the source of the peripheral envelope protein of cornified cells.

摘要

利用配备了能量色散X射线光谱仪的EMMA - 4分析电子显微镜，研究了新生大鼠和成年大鼠表皮中不同类型透明角质颗粒的元素组成。对透明角质颗粒中的硫浓度进行了绝对定量。结果表明，表皮透明角质颗粒在化学组成上是异质的。在新生大鼠和成年大鼠表皮细胞核和细胞质中均存在的一种透明角质颗粒——称为单颗粒——富含硫，含量为2 - 5 - 3 - 6%。新生大鼠和成年大鼠中不同的其他类型透明角质颗粒含有硫含量低的成分；它们通常也有富含硫的成分。硫含量低的透明角质含硫量为0 - 6 - 0 - 9%。有人认为，富含硫的透明角质颗粒是角质化细胞外周包膜蛋白的来源。