Garg D K, Ali R
Faculty of Medicine, A.M.U., Aligarh, 202002, India.
J Autoimmun. 1998 Aug;11(4):371-8. doi: 10.1006/jaut.1998.0208.
The effect of the hydroxyl radical on polyguanylic acid [poly(G)] was investigated with regard to progressive increase of autoantibodies against it in systemic lupus erythematosus (SLE) and progressive systemic sclerosis (PSS). Rabbits immunized with both native and ROS-poly(G) induced high titre antibodies. Immune IgG exhibited a high degree of specificity towards the immunogen, reiterated visually by a gel retardation assay. The induced antibodies showed a wide range of cross-reactivity with various synthetic polynucleotides exhibiting B-, A-, and allied conformations. The specificity of induced antibodies resembled the diverse binding characteristics of lupus anti-DNA autoantibodies. Moreover, sera from scleroderma patients showed binding to native and ROS-poly(G). SLE and PSS autoantibodies showed preferential recognition of ROS-poly(G) over native poly(G). These results demonstrate that the hydroxyl modified guanine residues in DNA and RNA can induce circulating SLE and PSS autoantibodies.
针对系统性红斑狼疮（SLE）和进行性系统性硬化症（PSS）中针对聚鸟苷酸[poly(G)]的自身抗体逐渐增加的情况，研究了羟基自由基对聚鸟苷酸的影响。用天然和ROS修饰的聚鸟苷酸免疫的兔子诱导出高滴度抗体。免疫IgG对免疫原表现出高度特异性，凝胶阻滞试验直观地重复显示了这一点。诱导产生的抗体与具有B型、A型和相关构象的各种合成多核苷酸表现出广泛的交叉反应性。诱导抗体的特异性类似于狼疮抗DNA自身抗体的多种结合特性。此外，硬皮病患者的血清显示与天然和ROS修饰的聚鸟苷酸结合。SLE和PSS自身抗体对ROS修饰的聚鸟苷酸的识别优于天然聚鸟苷酸。这些结果表明，DNA和RNA中羟基修饰的鸟嘌呤残基可诱导循环中的SLE和PSS自身抗体。
