San-Blas G, Moreno B, Calcagno A M, San-Blas F
Instituto Venezolano de Investigaciones Científicas (IVIC), Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020A, Venezuela.
Med Mycol. 1998 Apr;36(2):75-9.
Zygosporium geminatum, isolated as a contaminant in a culture of the mycelial phase of Paracoccidioides brasiliensis, was lethal to the latter organism. Its lytic action was due to exocellular alpha-1,3- and beta-1,3-glucanases which degraded the P. brasiliensis cell wall. The alpha-1,3-glucanase was more active at 30 degrees C and the beta-1,3-glucanase at 23 degrees C, each having pH 6.0 as its optimum.
双孢接合菌(Zygosporium geminatum)作为巴西副球孢子菌(Paracoccidioides brasiliensis)菌丝体阶段培养物中的污染物被分离出来,对后者具有致死性。其溶解作用归因于细胞外α-1,3-和β-1,3-葡聚糖酶,这些酶可降解巴西副球孢子菌的细胞壁。α-1,3-葡聚糖酶在30℃时活性更高,β-1,3-葡聚糖酶在23℃时活性更高,两者的最适pH均为6.0。