Graffeo A P, Lin D C, Foltz R L
J Chromatogr. 1976 Nov 3;126:717-22. doi: 10.1016/s0021-9673(01)84114-5.
Benzoylecgonine, a polar metabolite of cocaine, was extracted and purified from urine using a reversed-phase high-performance liquid chromatographic column. The portion of the eluent corresponding to elution of the drug was collected, derivatized, and quantified using gas chromatography-mass spectrometry with selected ion monitoring. Using this procedure, analysis of 1 ng/ml of benzoylecgonine in urine can be achieved.
可卡因的极性代谢物苯甲酰芽子碱，通过反相高效液相色谱柱从尿液中提取和纯化。收集与药物洗脱相对应的洗脱液部分，进行衍生化处理，并使用选择离子监测的气相色谱-质谱联用仪进行定量分析。采用该方法，可实现对尿液中1纳克/毫升苯甲酰芽子碱的分析。
