人髓母细胞瘤细胞的干扰素产量和MHC抗原表达及其在二丁酰环磷酸腺苷诱导分化过程中的抑制：髓母细胞瘤细胞是否源自双能神经元和神经胶质祖细胞？
Interferon yield and MHC antigen expression of human medulloblastoma cells and its suppression during dibutyryl cyclic AMP-induced differentiation: do medulloblastoma cells derive from bipotent neuronal and glial progenitors?
作者信息
Park K C, Shimizu K, Hayakawa T
机构信息
Institute for Molecular and Cellular Biology, Osaka University, Japan.
出版信息
Cell Mol Neurobiol. 1998 Oct;18(5):497-507. doi: 10.1023/a:1026327309345.
Abstract
- Human medulloblastoma (ONS-76), a central nervous system (CNS)-derived undifferentiated cell line, was found to possess glial characteristics as defined by responses in the interferon (IFN) system; ONS-76 cells produced as much IFN-beta as human fibroblast and glioma cells by viral infection and poly(I):poly(C) induction. 2. Major histocompatibility complex (MHC) class I antigens were also induced under IFN-beta stimulation. ONS-76 cells expressed neurofilament protein, as shown by Northern blot analysis, and morphological differentiation was induced by dibutyryl cyclic AMP (dcAMP). 3. Expression of IFN-beta and MHC class I antigens was suppressed in ONS-76 cells during the dcAMP-induced differentiation. 4. These results showed that ONS-76 cells possessed a glial property in IFN system responses and a neuronal property in cytoskeleton protein, suggesting that the precursors of medulloblastoma may be characterized as bipotent neuronal and glial progenitors in CNS.
摘要
- 人髓母细胞瘤（ONS-76）是一种源自中枢神经系统（CNS）的未分化细胞系，通过干扰素（IFN）系统中的反应定义发现其具有神经胶质细胞特征；ONS-76细胞经病毒感染和聚肌苷酸:聚胞苷酸（poly(I):poly(C)）诱导后产生的IFN-β与人类成纤维细胞和胶质瘤细胞一样多。2. 在IFN-β刺激下，主要组织相容性复合体（MHC）I类抗原也被诱导产生。如Northern印迹分析所示，ONS-76细胞表达神经丝蛋白，并且二丁酰环磷腺苷（dcAMP）可诱导其形态分化。3. 在dcAMP诱导的分化过程中，ONS-76细胞中IFN-β和MHC I类抗原的表达受到抑制。4. 这些结果表明，ONS-76细胞在IFN系统反应中具有神经胶质细胞特性，在细胞骨架蛋白方面具有神经元特性，提示髓母细胞瘤的前体细胞可能具有中枢神经系统中双能神经元和神经胶质祖细胞的特征。
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本文引用的文献
1
Anti-oncogenic and oncogenic potentials of interferon regulatory factors-1 and -2.
Science. 1993 Feb 12;259(5097):971-4. doi: 10.1126/science.8438157.
2
Cellular commitment to oncogene-induced transformation or apoptosis is dependent on the transcription factor IRF-1.
Cell. 1994 Jun 17;77(6):829-39. doi: 10.1016/0092-8674(94)90132-5.
3
An IRF-1-dependent pathway of DNA damage-induced apoptosis in mitogen-activated T lymphocytes.
Nature. 1995 Aug 17;376(6541):596-9. doi: 10.1038/376596a0.
4
Isolation, characterization, and use of stem cells from the CNS.
Annu Rev Neurosci. 1995;18:159-92. doi: 10.1146/annurev.ne.18.030195.001111.
5
Inducible expression of H-2 and Ia antigens on brain cells.
Nature. 1984;310(5979):688-91. doi: 10.1038/310688a0.
6
Genomic sequencing.
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
7
Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP.
Cell. 1980 Sep;21(2):347-55. doi: 10.1016/0092-8674(80)90471-7.
8
Interferon synthesis in the early post-implantation mouse embryo.
Differentiation. 1984;27(3):229-35. doi: 10.1111/j.1432-0436.1984.tb01433.x.
9
Studies on interferon priming: cellular response to viral and nonviral inducers and requirement of protein synthesis.
Virology. 1981 Jul 15;112(1):62-9. doi: 10.1016/0042-6822(81)90612-7.
10
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5. doi: 10.1073/pnas.77.9.5201.
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