伊朗多发性硬化症（MS）患者中与人类白细胞抗原II类（DRB1、DQA1和DQB1）相关的遗传易感性
HLA class II (DRB1, DQA1 and DQB1) associated genetic susceptibility in Iranian multiple sclerosis (MS) patients.
作者信息
Amirzargar A, Mytilineos J, Yousefipour A, Farjadian S, Scherer S, Opelz G, Ghaderi A
机构信息
Ahwaz University of Medical Sciences, Iran.
出版信息
Eur J Immunogenet. 1998 Aug;25(4):297-301. doi: 10.1046/j.1365-2370.1998.00101.x.
The association of HLA class II alleles with multiple sclerosis (MS) has been amply documented. In the present study, the role of HLA class II (DRB1, DQA1 and DQB1) alleles and haplotypes was investigated in 43 unrelated Iranian chronic progressive multiple sclerosis (CP-MS) patients compared with 100 healthy individuals. HLA typing for DRB1, DQA1 and DQB1 was performed by restriction fragment length polymorphism (RFLP). Subtypes of DR4, DR15 and DR16 were defined using polymerase chain reaction (PCR) amplification with sequence-specific primers (PCR-SSP). The results show that, among DR2-positive MS patients and the control group, a positive association with the DRB11503, DQA10102, DQB10602 haplotype (21% vs. 2.7%, P = 0.057, RR = 9.8) and a negative association with the most frequent DR15 haplotype in the control group, DRB115021, DQA10103, DQB10601 (7% vs. 24.3%, P = 0.001), were observed. No significant association was found with the analysed HLA-DRB1, DQA1 and DQB1 alleles.
HLA Ⅱ类等位基因与多发性硬化症（MS）之间的关联已有大量文献记载。在本研究中，对43例无亲缘关系的伊朗慢性进展型多发性硬化症（CP-MS）患者与100名健康个体进行了研究，以探讨HLA Ⅱ类（DRB1、DQA1和DQB1）等位基因及单倍型的作用。采用限制性片段长度多态性（RFLP）技术对DRB1、DQA1和DQB1进行HLA分型。使用序列特异性引物聚合酶链反应（PCR-SSP）对DR4、DR15和DR16的亚型进行定义。结果显示，在DR2阳性的MS患者和对照组中，观察到与DRB11503、DQA10102、DQB10602单倍型呈正相关（21%对2.7%，P = 0.057，RR = 9.8），而与对照组中最常见的DR15单倍型DRB115021、DQA10103、DQB10601呈负相关（7%对24.3%，P = 0.001）。在分析的HLA-DRB1、DQA1和DQB1等位基因中未发现显著关联。
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