Nölting B
Prussian Private Institute of Technology at Berlin, Görschstr 40, D-13187 Berlin, Germany.
J Theor Biol. 1998 Oct 7;194(3):419-28. doi: 10.1006/jtbi.1998.0783.
Folding of barstar, the 10 kDalton inhibitor of the ribonuclease barnase, has been suggested to follow a nucleation-condensation model [Nölting, B., Golbik, R., Neira, J. L., Soler-Gonzalez, A. S., Schreiber, G. & Fersht, A. R. (1997). Proc. Nat. Acad. Sci. U.S.A. 94, 826-830], where structure growth starts in a particular region of the molecule, the folding nucleus. Here the structure of the diffuse nucleus and its growth in three stages, 500 micros, 1 ms and 100 ms after initiation of the folding reaction, is mapped out by using phi-values which are correlate with inter-residue contact plots. Barstar folding is initiated by a significant consolidation of interactions in and around the strand1-loop1-helix1 motif in the microsecond time scale, followed by the consolidation of helix4, which is located close to the C-terminus and does not have significant residual structure in the cold-denatured state. The non-uniform structure consolidation is most pronounced in the early stages of folding. The late folding events of barstar are characterized by a propagation of structure consolidation from the N-and C-termini towards residues located in the center of the sequence.
核糖核酸酶barnase的10千道尔顿抑制剂barstar的折叠，被认为遵循成核-凝聚模型【诺尔廷，B.，戈尔比克，R.，内拉，J. L.，索勒-冈萨雷斯，A. S.，施赖伯，G. & 弗什特，A. R.（1997年）。《美国国家科学院院刊》94，826 - 830】，其中结构生长始于分子的特定区域，即折叠核。在此，通过使用与残基间接触图相关的φ值，描绘了折叠反应开始后500微秒、1毫秒和100毫秒这三个阶段中扩散核的结构及其生长情况。Barstar折叠在微秒时间尺度上由strand1-loop1-helix1基序及其周围相互作用的显著巩固引发，随后是靠近C端且在冷变性状态下没有显著残余结构的螺旋4的巩固。结构巩固的不均匀性在折叠早期最为明显。Barstar的后期折叠事件的特征是结构巩固从N端和C端向序列中心的残基传播。
