Chouljenko V N, Kousoulas K G, Lin X, Storz J
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA.
Virus Genes. 1998;17(1):33-42. doi: 10.1023/a:1008048916808.
The 3'-ends of the genomes (9538 bp) of two wild-type respiratory bovine coronavirus (RBCV) isolates LSU and OK were obtained by cDNA sequencing. In addition, the 3'-end of the genome (9545) of the wild-type enteric bovine coronavirus (EBCV) strain LY-138 was assembled from available sequences and by cDNA sequencing of unknown genomic regions. Comparative analyses of RBCV and EBCV nucleotide and deduced amino acid sequences revealed that RBCV-specific nucleotide and amino acid differences were disproportionally concentrated within the S gene and the genomic region between the S and E genes. Comparisons among virulent and avirulent BCV strains revealed that virulence-specific nucleotide and amino acid changes were located within the S and E genes, and the 32 kDa open reading frame.
通过cDNA测序获得了两株野生型呼吸道牛冠状病毒（RBCV）分离株LSU和OK的基因组（9538 bp）的3'末端。此外，野生型肠道牛冠状病毒（EBCV）毒株LY-138的基因组3'末端（9545）是根据可用序列以及未知基因组区域的cDNA测序组装而成。对RBCV和EBCV核苷酸及推导氨基酸序列的比较分析表明，RBCV特异性的核苷酸和氨基酸差异不成比例地集中在S基因以及S基因和E基因之间的基因组区域内。强毒和无毒BCV毒株之间的比较显示，毒力特异性核苷酸和氨基酸变化位于S基因、E基因以及32 kDa开放阅读框内。
