Massa M, Pignatti P, Oliveri M, De Amici M, De Benedetti F, Martini A
Laboratorio Biotecnologie e Tecnologie Biomediche, Università degli Studi di Pavia, I.R.C.C.S. Policlinico San Matteo, Italy.
Clin Exp Rheumatol. 1998 Sep-Oct;16(5):611-6.
To assess the CD23 status in patients with juvenile chronic arthritis (JCA), as defined by serum soluble CD23 (sCD23) and the expression of CD23 on peripheral blood mononuclear cells.
Serum sCD23 levels were measured by ELISA in 22 patients with systemic JCA (s-JCA), in 40 patients with antinuclear antibody positive pauciarticular JCA (ANA+ p-JCA), and in 38 healthy controls. CD23 expression on T cells, B cells, and monocytes was determined by flow cytometry analysis of double fluorescence staining.
Serum sCD23 levels were increased in both ANA+ p-JCA and s-JCA; no relation with disease activity or severity was found. In patients with ANA+ p-JCA, serum sCD23 levels were correlated with an increased percentage of B cells expressing CD23, while in patients with s-JCA the serum sCD23 levels were correlated with an increased percentage of monocytes expressing CD23.
Serum sCD23 levels are elevated both in systemic and ANA+ pauciarticular JCA: different cell subset CD23 expression in s-JCA and ANA+ p-JCA (i.e. monocyte or B cell, respectively) suggests that in pauciarticular JCA CD23 may be implicated in B cell activation and autoantibody production, while in systemic JCA may be involved in monocyte activation and in the release of inflammatory mediators.
通过血清可溶性CD23（sCD23）及外周血单个核细胞上CD23的表达来评估青少年慢性关节炎（JCA）患者的CD23状态。
采用酶联免疫吸附测定法（ELISA）检测22例全身型JCA（s-JCA）患者、40例抗核抗体阳性少关节型JCA（ANA+ p-JCA）患者及38名健康对照者的血清sCD23水平。通过双荧光染色的流式细胞术分析测定T细胞、B细胞及单核细胞上CD23的表达。
ANA+ p-JCA和s-JCA患者的血清sCD23水平均升高；未发现其与疾病活动度或严重程度相关。在ANA+ p-JCA患者中，血清sCD23水平与表达CD23的B细胞百分比增加相关，而在s-JCA患者中，血清sCD23水平与表达CD23的单核细胞百分比增加相关。
全身型和ANA+少关节型JCA患者的血清sCD23水平均升高：s-JCA和ANA+ p-JCA中不同细胞亚群的CD23表达（分别为单核细胞或B细胞）表明，在少关节型JCA中CD23可能与B细胞活化及自身抗体产生有关，而在全身型JCA中可能参与单核细胞活化及炎症介质的释放。
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