Cytokeratin immunostaining in normal dog major and minor salivary glands.

Immunohistochemical staining, with a monoclonal antibody specific for cytokeratins 5, 6, 8, 17 and 19 of dog submandibular, sublingual, parotid, palatine, tongue and zygomatic salivary glands resulted in staining of myoepithelial cells, duct epithelial cells (including intercalated, striated and secretory ducts) and some acinar epithelial cells. Differences in acinar staining between glands reflected the predominance of serous or mucous cells. Acinar epithelial staining was confined to serous cells in sublingual and zygomatic glands and in some parotid glands; serous cells in other parotid glands, and serous demilunes in submandibular, tongue and palatine glands, were unstained. Mucous cells were not stained in any of the glands.