Ferko B, Katinger D, Grassauer A, Egorov A, Romanova J, Niebler B, Katinger H, Muster T
Institut für Angewandte Mikrobiologie, Universität für Bodenkultur, Vienna, Austria.
J Infect Dis. 1998 Nov;178(5):1359-68. doi: 10.1086/314445.
Previously, a mucosal model of immunization against human immunodeficiency virus type 1 (HIV-1) was established by using influenza virus as a vector for the neutralizing gp41 epitope ELDKWA. Whether replication of this chimeric influenza virus in the upper respiratory tract of mice is sufficient for inducing mucosal immune responses in the genital tract was investigated. An immunization strategy was established that permits the virus to replicate in the murine upper respiratory tracts but not in the lungs. Intranasal application of the chimeric virus induced HIV-1-specific antibodies in sera and genital tract. In addition, chimeric virus-specific antibody-secreting cells were detected in lymphocyte populations obtained from lungs, spleens, and urogenital tracts. These results indicate that replication of the chimeric influenza/ELDKWA virus in the upper respiratory tract is sufficient to induce systemic immune responses as well as local immune responses in the genital tract.
此前，通过使用流感病毒作为中和性gp41表位ELDKWA的载体，建立了针对1型人类免疫缺陷病毒（HIV-1）的黏膜免疫模型。研究了这种嵌合流感病毒在小鼠上呼吸道中的复制是否足以诱导生殖道中的黏膜免疫反应。建立了一种免疫策略，使病毒能够在小鼠上呼吸道中复制，但不在肺部复制。鼻内接种嵌合病毒可诱导血清和生殖道中产生HIV-1特异性抗体。此外，在从肺、脾和泌尿生殖道获得的淋巴细胞群体中检测到了嵌合病毒特异性抗体分泌细胞。这些结果表明，嵌合流感/ELDKWA病毒在上呼吸道中的复制足以诱导全身免疫反应以及生殖道中的局部免疫反应。
