Schwander S K, Torres M, Sada E, Carranza C, Ramos E, Tary-Lehmann M, Wallis R S, Sierra J, Rich E A
Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
J Infect Dis. 1998 Nov;178(5):1434-45. doi: 10.1086/314454.
Responses to mycobacterial and nonmycobacterial antigens were examined in bronchoalveolar cells (BAC) and peripheral blood mononuclear cells (PBMC) from patients with active pulmonary tuberculosis (n=16) and healthy subjects (n=23). DNA synthesis in BAC (but not PBMC) from tuberculosis patients was significantly increased in response to the mycobacterial antigens purified protein derivative (PPD), antigen 85, and mannose-capped lipoarabinomannan but not to nonmycobacterial antigens. The response to PPD was also increased in enriched alveolar lymphocytes from tuberculosis patients (P<.05). The frequency of interferon-gamma but not interleukin-4- or -10-producing cells by ELISAspot was increased in PPD-stimulated BAC from patients with tuberculosis (P<.05). Accessory function of alveolar macrophages for T lymphocyte responses was similar and suppressive activity was variably decreased in tuberculosis patients. Thus, there is compartmentalization of mycobacterial antigen-specific lymphocytes to the lungs during active tuberculosis that on challenge produce a Th1-type cytokine host response.
在活动性肺结核患者（n = 16）和健康受试者（n = 23）的支气管肺泡细胞（BAC）和外周血单核细胞（PBMC）中检测了对分枝杆菌和非分枝杆菌抗原的反应。肺结核患者的BAC（而非PBMC）中的DNA合成在对分枝杆菌抗原纯化蛋白衍生物（PPD）、抗原85和甘露糖封端的脂阿拉伯甘露聚糖反应时显著增加，但对非分枝杆菌抗原无反应。来自肺结核患者的富集肺泡淋巴细胞对PPD的反应也增加（P<0.05）。通过酶联免疫斑点法检测，肺结核患者的PPD刺激的BAC中产生干扰素-γ而非白细胞介素-4或-10的细胞频率增加（P<0.05）。肺泡巨噬细胞对T淋巴细胞反应的辅助功能相似，且肺结核患者的抑制活性有不同程度降低。因此，在活动性肺结核期间，分枝杆菌抗原特异性淋巴细胞定位于肺部，受到刺激时产生Th1型细胞因子宿主反应。
