[Simple determination methods for blood grouping from biological stains. 2. Immunochromatographic determination of ABO blood groups from saliva specimens].

A simple immunochromatographic method was used to determine ABO blood groups and secretor status from saliva stains. Saliva specimens were serially diluted with PBS and dotted onto nitrocellulose strips (10 mm x 70-100 mm). One end of the strips was dipped in two drops of 0.1% orange-red gold particles (GP) which were coated with an anti-A, anti-B or anti-H blood group reagent. The GP migrated along the surface of the strips producing red spots where positive antigen-antibody reactions occurred. In non-secretors, the end-points of saliva dilution were lower than in secretors. The results of this test were obtained within 2 to 3 minutes. Moreover, the GP reagents utilized were quite inexpensive. The principles underlying this method might be useful in species and organ identification.